Expressed sequence tags (ESTs) have been obtained from eighteen species of plant pathogenic fungi, two species of phytopathogenic oomycete and three species of saprophytic fungi. Hierarchical clustering software was used to classify together ESTs representing the same gene and produce a single contig, or consensus sequence. The unisequence set for each pathogen therefore represents a set of unique gene sequences, each one consisting of either a single EST or a contig sequence made from a group of ESTs. Unisequences were annotated based on top hits against the NCBI non-redundant protein database using blastx.
This is an ongoing BBSRC-funded study to construct a relational database of genomic information from phytopathgenic fungi as part of the COGEME project.References: Soanes, D.M., Talbot, N.J. (2006) Comparative genomic analysis of phytopathogenic fungi using expressed sequence tag (EST) collections. Molecular Plant Pathology 7: 61-70. [pdf]
Soanes, D.M., Skinner, W., Keon, J., Hargreaves, J., Talbot, N.J. (2002) Genomics of phytopathogenic fungi and the development of bioinformatic resources. Molecular Plant-Microbe Interactions 15: 421-427. [pdf]
This site also hosts microarray data for Blumeria graminis provided by Maike Both and Pietro Spanu at Imperial College, London.
There is a mirror of the COGEME database, kindly hosted by the Institute for Marine Biosciences Research Informatics and maintained by Sheldon Briand.Database and website constructed and maintained by Darren Soanes (University of Exeter)