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   Transcript abundance
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Search against putative product / function

This form enables you to query the putative protein products / functions of the unisequences in the database. Enter a search term in the text box and select whether you want to query the whole set of unisequences or just unisequences from one organism. You do not have to match the protein product / function exactly. The query will return all unisequences that contain the search term in their putative protein products / function field. For example, putting "glucose" in the search term will return glucose transporters as well as enzymes such as glucose-6-phosphate isomerase. You can use "%" as a wildcard character. For example, putting "vacuolar%ATPase" in the search term will return all vacuolar ATPases, including those annotated as "vacuolar membrane ATPase" and "vacuolar H+ -ATPase". Press the Submit button to run the query. Press the Reset button to clear the form. The query produes a table showing unisequences, the EST set they are from and their putative protein product / function. Click on the name of the unisequence for further information about it. Click on "Retrieve sequences" to open a new browser window containing a FASTA formatted file with all the sequences from the query.

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Search against top hits

The blastx algorithim was used to compare unisequences in this database with the non-redundant database at NCBI. The database contains details of the top twenty hits (with an E-value of less than 10-5). This form can be used to query the hits. Entering a search term in the text box enables you to query the definitions for each hit (i.e. name of protein product). You do not have to match the definition exactly. The query will return all hits that contain the search term in their definition field. The definition contains accession number and species as well as annotation. For example putting "glucose" in the search term will return glucose transporters as well as enzymes such as glucose-6-phosphate isomerase. You can use "%" as a wildcard character. You can also select whether you want to query the whole set of unisequences or just unisequences from one organism. Press the Submit button to run the query. Press the Reset button to clear the form. The query produces a table showing details of the hits and unisequences they have similarities to. Click on the name of the unisequence for further information about it. Click on the hit definition for a link to its entry in the NCBI database